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KMID : 0545120120220101324
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Journal of Microbiology and Biotechnology
2012 Volume.22 No. 10 p.1324 ~ p.1329
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Increased Sensitivity to Chloramphenicol by Inactivation of manB in Streptomyces coelicolor
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Thangamani Rajesh
Song Eun-Jung
Lee Bo-Rahm
Park Sung-Hee
Jeon Jong-Min
Kim Eun-Jung
Sung Chang-Min
Lee Jae-Hun
Yoo Dong-Won
Park Hyung-Yeon
Kim Yun-Gon
Kim Byung-Gee
Yang Yung-Hun
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Abstract
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Phosphomannomutase (ManB) is involved in the biosynthesis of GDP-mannose, which is vital for numerous processes such as synthesis of carbohydrates, production of alginates and ascorbic acid, and post-translational modification of proteins. Here, we discovered that a deletion mutant of manB (BG101) in Streptomyces coelicolor (S. coelicolor) showed higher sensitivity to bacteriostatic chloramphenicol (CM) than the wild-type strain (M145), along with decreased production of CM metabolites. Deletion of manB also decreased the mRNA expression level of drug efflux pumps (i.e., cmlR1 and cmlR2) in S. coelicolor, resulting in increased sensitivity to CM. This is the first report on changes in antibiotic sensitivity to CM by deletion of one glycolysis-related enzyme in S. coelicolor, and the results suggest different approaches for studying the antibioticresistant mechanism and its regulation.
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KEYWORD
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Streptomyces coelicolor, phosphomannomutase, manB, antibiotic resistance, chloramphenicol, mannosylation
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